| 產(chǎn)品編號 | bsm-33170M-Bio |
| 英文名稱 | Vimentin |
| 中文名稱 | Biotin標記的波形蛋白單克隆抗體 |
| 別 名 | FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin. |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 3B7 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Vimentin Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Vimentin monoclonal antibody, Biotin conjugated (bsm-33170M-Bio) at 1:1000 dilution and 4°C overnight incubation. Followed by HRP conjugated Streptavidin (bs-0437P-HRP) incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4°C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
