| 產(chǎn)品編號 | bsm-60265M |
| 英文名稱 | HDAC3 Mouse mAb |
| 中文名稱 | 組蛋白去乙?；?單克隆抗體 |
| 別 名 | HDAC3_HUMAN; Histone deacetylase 3; EC:3.5.1.98; HD3; Protein deacetylase HDAC3; EC:3.5.1.; Protein deacylase HDAC3; RPD3-2; SMAP45; SMAP 45; SMAP-45; RPD3; KDAC3; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C12C4 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG2a, Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 負責核心組蛋白（H2A、H2B、H3和H4）N端部分和其他一些非組蛋白底物上賴氨酸殘基的脫乙酰。組蛋白去乙?；癁楸碛^遺傳抑制提供了一個標簽，在轉(zhuǎn)錄調(diào)控、細胞周期進展和發(fā)育事件中起著重要作用。組蛋白脫乙?；竿ㄟ^形成大的多蛋白復合物起作用。通過使增強子元件上的H3'Lys-27'（H3K27）去乙?；?，拮抗EP300乙酰轉(zhuǎn)移酶活性和抑制近端基因表達，參與BCL6轉(zhuǎn)錄抑制因子的活性 |
| 產(chǎn)品圖片 |
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60265M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
