| 產(chǎn)品編號 | bsm-60209R |
| 英文名稱 | 2-Hydroxyisobutyryl-Histone H3 (Lys14) Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B3E9 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白受到多種酶催化修飾，包括乙?；?、甲基化、磷酸化和泛素化等。賴氨酸2-羥基異丁基?；且环N新發(fā)現(xiàn)的控制染色體結(jié)構(gòu)和基因轉(zhuǎn)錄的可逆修飾，其可逆性已在從蠕蟲到人類的真核組蛋白中得以證實。組蛋白2-羥基異丁基?；莫毺亟Y(jié)構(gòu)和基因組定位表明，它在機制和功能上與組蛋白乙酰化有所不同。具體而言，在人類體細胞和小鼠雄性生殖細胞基因組中，組蛋白2-羥基異丁?；瘶?biāo)記了活性啟動子或潛在的增強子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
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