產品編號 | bs-19336R |
英文名稱 | Nucleoplasmin 3 Rabbit pAb |
中文名稱 | 核仁磷酸蛋白3抗體 |
別 名 | NPM 3; NPM3; NPM3_HUMAN; Nub1; nucleophosmin/nucleoplasmin family, member 3; nucleophosmin/nucleoplasmin, 3; Nucleoplasmin 3; Nucleoplasmin-3; PORMIN; RGD1565542; TMEM123. |
研究領域 | 細胞生物 信號轉導 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 19 kDa |
檢測分子量 | |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Nucleoplasmin 3: 2-100/178 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
The protein encoded by this gene is related to the nuclear chaperone phosphoproteins, nucleoplasmin and nucleophosmin. This protein is strongly expressed in diverse cell types where it localizes primarily to the nucleus. Based on its similarity to nucleoplasmin and nucleophosmin, this protein likely functions as a molecular chaperone in the cell nucleus. [provided by RefSeq, Oct 2008] Function: May act as a chaperone. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated. Similarity: Belongs to the nucleoplasmin family. SWISS: O75607 Gene ID: 10360 Database links: Entrez Gene: 10360 Human Entrez Gene: 18150 Mouse Omim: 606456 Human SwissProt: O75607 Human SwissProt: Q9CPP0 Mouse Unigene: 90691 Human Unigene: 1406 Mouse |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nucleoplasmin 3) Polyclonal Antibody, Unconjugated (bs-19336R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |