| 產品編號 | bs-19010R |
| 英文名稱 | NAP1/C12orf30 Rabbit pAb |
| 中文名稱 | 12號染色體開放閱讀框30抗體 |
| 別 名 | C12orf30; DKFZp667K2112; FLJ1308; MDM20; Mitochondrial distribution and morphology protein 20; N(alpha)-acetyltransferase 25, NatB auxiliary subunit; N-alpha-acetyltransferase 25, NatB auxiliary subunit; N-terminal acetyltransferase B complex subunit MDM20; N-terminal acetyltransferase B complex subunit NAA25; Naa25; NAA25_HUMAN; NAP1; NatB complex subunit MDM20; p120; TPR repeat-containing protein C12orf30. |
| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 112 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NAP1/C12orf30: 51-150/972 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes the auxiliary subunit of the heteromeric N-terminal acetyltransferase B complex. This complex acetylates methionine residues that are followed by acidic or asparagine residues.[provided by RefSeq, Mar 2010] Function: Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression. Subunit: Component of the N-terminal acetyltransferase B (NatB) complex which is composed of NAA20 and NAA25. Subcellular Location: Cytoplasm. Similarity: Belongs to the MDM20/NAA25 family. Contains 4 TPR repeats. SWISS: Q14CX7 Gene ID: 80018 Database links: Entrez Gene: 80018 Human Entrez Gene: 231713 Mouse Omim: 612755 Human SwissProt: Q14CX7 Human SwissProt: Q8BWZ3 Mouse Unigene: 530941 Human Unigene: 33919 Mouse Unigene: 202650 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NAP1) Polyclonal Antibody, Unconjugated (bs-19010R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
