| 產(chǎn)品編號 | bs-18715R |
| 英文名稱 | MCART1 Rabbit pAb |
| 中文名稱 | MCART1蛋白抗體 |
| 別 名 | CG7943; FLJ37273; SLC25A51; Solute carrier family 25 member 51; MCAR1_HUMAN; MCART 1; MCART1; MGC14836; Mitochondrial carrier triple repeat 1; Mitochondrial carrier triple repeat protein 1; OTTHUMP00000195034. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCART1: 61-160/297 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MCARTs (mitochondrial carrier triple repeat protein) are mitochondrial multipass inner membrane proteins that contain three solcar repeats, which are typical of substrate carrier proteins involved in energy transfer. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: Q9H1U9 Gene ID: 92014 Database links: Entrez Gene: 92014 Human Entrez Gene: 230125 Mouse SwissProt: Q9H1U9 Human SwissProt: Q5HZI9 Mouse Unigene: 645492 Human Unigene: 260210 Mouse |
| 產(chǎn)品圖片 |
Sample:
293FT(Human) Cell Lysate at 30 ug
Primary: Anti-MCART1 (bs-18715R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCART1) Polyclonal Antibody, Unconjugated (bs-18715R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
