| 產(chǎn)品編號 | bsm-62995R |
| 英文名稱 | Thyroid peroxidase Recombinant Rabbit mAb |
| 中文名稱 | 甲狀腺過氧化物酶重組兔單抗 |
| 別 名 | MSA; TDH2A; TPO; TPX. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 13H17 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 103 kDa |
| 檢測分子量 | 103 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Thyroid peroxidase: 880-933 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4. SWISS: P07202 Gene ID: 7173 甲狀腺過氧化物酶TPO是一種含鐵卟啉的血紅蛋白類物質，位于甲狀腺上皮細胞的頂端細胞膜上。 TPO主要的生物學作用是使碘活化，酪氨酸碘化和促進碘化酪氨酸的耦聯(lián)，在甲狀腺激素合成過程中起著關鍵作用-是甲狀腺激素合成過程中的關鍵酶. |
| 產(chǎn)品圖片 |
Western blot analysis of Human thyroid lysate. Using Thyroid peroxidase (bsm-62995R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human thyroid gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(bsm-62995R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
（Negative samples）Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PKM2 Monoclonal Antibody, Unconjugated(bsm-62995R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
