| 產(chǎn)品編號(hào) | bsm-62314R |
| 英文名稱 | FXR1 Recombinant Rabbit mAb |
| 中文名稱 | 脆性X相關(guān)蛋白1/脆性X智力低下綜合征相關(guān)蛋白1重組兔單抗 |
| 別 名 | FXR1; FXR1P; hFXR1p. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 9D20 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | 70-85 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human FXR1: 4-63/621 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. SWISS: P51114 Gene ID: 8087 脆性X綜合癥，又稱馬?。悹柧C合癥，是一種遺傳疾病。該綜合癥可以導(dǎo)致一系列的特征性癥狀，包括生理、智力、情緒、以及行為上的異常。癥狀的輕重各有不同。該疾病伴隨著X染色體上一個(gè)簡單的三核苷酸基因序列（CGG）的擴(kuò)增。這種擴(kuò)增導(dǎo)致了一種稱為FMR-1的蛋白質(zhì)無法在病人體內(nèi)表達(dá)，而該蛋白質(zhì)是神經(jīng)的正常發(fā)育必不可少的。 根據(jù)CGG重復(fù)序列的長度，目前普遍認(rèn)可將脆性X綜合癥分為四種類型：正常人（含有19－31個(gè)CGG重復(fù)序列），前突變者（含有55－200個(gè)CGG重復(fù)序列），全突變者（含有200個(gè)以上的CGG重復(fù)序列 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with FXR1 monoclonal antibody, unconjugated (bsm-62314R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FXR1) monoclonal Antibody, unconjugated (bsm-62314R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-FXR1 antibody (bsm-62314R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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