產(chǎn)品編號(hào) | bsm-62236R |
英文名稱 | KIF4A Recombinant Rabbit mAb |
中文名稱 | 沉默驅(qū)動(dòng)蛋白KIF4A重組兔單抗 |
別 名 | Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號(hào) | 1A12 |
交叉反應(yīng) | Human,Mouse |
產(chǎn)品應(yīng)用 | WB=1:500-1:2000,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 140 kDa |
檢測(cè)分子量 | 150 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human Chromokinesin A: 40-70/1232 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. SWISS: O95239 Gene ID: 24137 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with KIF4A monoclonal antibody, unconjugated (bsm-62236R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (KIF4A) monoclonal Antibody, unconjugated (bsm-62236R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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