| 產(chǎn)品編號 | bsm-62216R |
| 英文名稱 | CHRM2 Recombinant Rabbit mAb |
| 中文名稱 | 毒蕈堿型乙酰膽堿受體重組兔單抗 |
| 別 名 | CHRM2; HM2; AChR; Acm2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 12G9 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | 52 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CHRM2: 152-180/466 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. SWISS: P08172 Gene ID: 1129 ChRM2 ( muscarinic acetylcholine receptor M2 ) 毒蕈堿型乙酰膽堿受體通過與G蛋白偶聯(lián)，調(diào)節(jié)各種細胞應答，包括抑制腺苷酸環(huán)化酶，降解磷酸肌醇和調(diào)節(jié)鉀通道。 毒蕈堿型乙酰膽堿受體（又稱：Cholinergic receptor,mucarinic 2）是一類可以被毒蕈堿激活而受阿托品抑制的受體.此抗體真對Cholinergic receptor,mucarinic 2蛋白的N-端部分。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CHRM2 monoclonal antibody, unconjugated (bsm-62216R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
