| 產(chǎn)品編號 | bsm-62165R |
| 英文名稱 | Pokemon/ZBTB7A Recombinant Rabbit mAb |
| 中文名稱 | 撲克蒙蛋白重組兔單抗 |
| 別 名 | FBI1; LRF; Pokemon; TIP21; ZBTB7; ZBTB7A; ZNF857A. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2B1 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61 kDa |
| 檢測分子量 | 70 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ZBTB7A: 520-584 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. SWISS: O95365 Gene ID: 51341 Pokemon(7APOK Erythroid Myeloid Ontogenic factor)能夠控制將正常細胞變成癌細胞所需的途徑,Pokemon蛋白是轉(zhuǎn)錄因子蛋白家族的一員，并且在人類癌癥中發(fā)生了突變。另外Pokemon蛋白作為轉(zhuǎn)錄因子參與一些細胞基因轉(zhuǎn)錄的調(diào)節(jié),并在細胞分化過程中發(fā)揮著關(guān)鍵、多效性的功能。近來發(fā)現(xiàn),Poke-mon在致癌轉(zhuǎn)化過程中發(fā)揮著至關(guān)重要的功能,并與腫瘤的發(fā)生密切相關(guān)。 這種蛋白很可能在實質(zhì)腫瘤中處于重要地位,還有學(xué)者認(rèn)為：Pokemon在一定類型的B細胞和T細胞淋巴瘤中水 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Pokemon/ZBTB7A monoclonal antibody, unconjugated (bsm-62165R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A? Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A? Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A? Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A? Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A? Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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