| 產(chǎn)品編號 | bsm-62023R |
| 英文名稱 | CaMKI Recombinant Rabbit mAb |
| 中文名稱 | 鈣/鈣調(diào)蛋白依賴性蛋白激酶 I重組兔單抗 |
| 別 名 | CaM K1; CaM KI; CaMK 1; CAMK I; CaMK1 alpha; CAMK1 PEN; CaMKI alpha. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1A5 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | 41 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CAMKI: 45682 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. SWISS: Q14012 Gene ID: 8536 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CaMKI monoclonal antibody, unconjugated (bsm-62023R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CaMKI Monoclonal Antibody, Unconjugated(bsm-62023R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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