產(chǎn)品編號 | bsm-61887R |
英文名稱 | Band3 Recombinant Rabbit mAb |
中文名稱 | 紅細胞陰離子交換蛋白1重組兔單抗 |
別 名 | AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SLC4A1; SW; WD; WD1; WR. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | |
交叉反應 | Human |
產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 102 kDa |
檢測分子量 | 102 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human SLC4A1: 20-70/911 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein SWISS: P02730 Gene ID: 6521 |
產(chǎn)品圖片 |
Western blot analysis of Red blood cell lysate. Using Band3 (bsm-61887R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Band3 Monoclonal Antibody, Unconjugated (bsm-61887R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Band3 Monoclonal Antibody, Unconjugated (bsm-61887R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Band3 Monoclonal Antibody, Unconjugated (bsm-61887R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |